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Comparison of three different methods for radiolabelling human activated T lymphocytes
Authors:C Botti  DR Negri  E Seregni  V Ramakrishna  F Arienti  L Maffioli  C Lombardo  A Bogni  C Pascali  F Crippa  S Massaron  F Remonti  S Nerini-Molteni  S Canevari  E Bombardieri
Affiliation:Department of Social & Preventive Medicine, Graduate School of Medicine, Kyoto University, Japan.
Abstract:The purpose of this study was to determine both the selenium (Se) level and glutathione peroxidase (GSH-Px) activity in plasma, erythrocytes and platelets from 51 healthy Japanese individuals. The Se levels (mean +/- SD) of plasma, erythrocytes and platelets were 117.4 +/- 15.7 micrograms/L, 0.954 +/- 0.159 microgram/g hemoglobin, and 4.93 +/- 1.52 ng/mg protein, respectively, and GSH-Px activity was 318 +/- 50 U/L, 18.0 +/- 5.0 U/g hemoglobin, and 0.142 +/- 0.035 U/mg protein, respectively. There was a negative correlation between age and the platelet Se level in men (r = -0.761, p < 0.001), and a positive correlation between the plasma and platelet GSH-Px activities in women (r = 0.663, p < 0.001).
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