激光辐照对不同饵料微藻生长的影响

利用Nd:YAG激光(波长1.06μm,功率5W,照射时间0.5-3min)和Ar 激光(波长:488nm,功率70mw,照射时间5-20min)分别照射角毛藻和叉鞭金藻,辐照后进行细胞增殖和生长速率的测定。实验结果表明:照射剂量为1min的Nd:YAG激光及剂量为5min的Ar 激光对叉鞭金藻有较明显的促长效果,这两种激光处理组的细胞增殖量在辐照后的两天内较对照组分别高42.9%和48.1%,但这种促长效果随时间的推移逐渐消失。不同剂量的两种激光辐照角毛藻后,在延滞期均出现生长抑制现象,但进入指数生长期或传代培养后,剂量为1min的Nd:YAG激光及剂量为10min的Ar 激光对角毛藻有明显的促长效果,其中在指数生长期1min剂量的Nd:YAG激光处理可促长27.0%,传代培养后10min的Ar 激光处理组生长速率提高达51.2%。本文对不同激光及不同激光参数条件下,两种饵料藻的耐受性及生长特性的差异也进行分析探讨。本实验结果不仅证实了已有研究的推论,还展示出激光技术在饵料藻种选育中的应用前景。

Growth Effect of Laser on Different Bait-Microalgae

Nd:YAG laser(wavelength 1.06μm, power 5w,radiation time 0.5-3min)and Ar +(wavelength 488nm,power 70mv, radiation time 5-20min) laser were used to irradiate two kinds of bait-microalgae- Chaetoceros calcitrorns and Dicraterpia sp. The proliferation and growth rates of irradiated cells were determined. The experimental result shows that the growth of Dicrateria sp was promoted after the irradiation of 1min Nd:YAG and 5min Ar + laser. The irradiated cell proliferation was promoted by 42.9% and 48.1% in two days, but the improving effects faded away as time going by. The growth was inhibited after the irradiation of different radiation time in lag phase, but the growth of C.calcitrorns was promoted after the irradiation of 1min Nd:YAG and 10min Ar+ laser in exponential phase and generation. Among these, the growth rate was promoted by 27.0% after 1min Nd:YAG irradiation and by 51.2 after 10min Ar+ laser irradiation. The different endurance and growth features were compared and analyzed among different lasers and parameters.The result proved sonclusions from premises and reveal the prospect of appliance of lasers in bait-microalgae selection.