High-Fat Diet Impairs Muscle Function and Increases the Risk of Environmental Heatstroke in Mice |
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Authors: | Matteo Serano Cecilia Paolini Antonio Michelucci Laura Pietrangelo Flavia A Guarnier Feliciano Protasi |
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Affiliation: | 1.CAST, Center for Advanced Studies and Technology, University G. D’Annunzio of Chieti-Pescara, 66100 Chieti, Italy; (M.S.); (C.P.); (A.M.); (L.P.);2.DMSI, Department of Medicine and Aging Sciences, University G. D’Annunzio of Chieti-Pescara, 66100 Chieti, Italy;3.DNICS, Department of Neuroscience and Clinical Sciences, University G. D’Annunzio of Chieti-Pescara, 66100 Chieti, Italy;4.Department of General Pathology, Londrina State University, Londrina 86057-970, Brazil; |
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Abstract: | Environmental heat-stroke (HS) is a life-threatening response often triggered by hot and humid weather. Several lines of evidence indicate that HS is caused by excessive heat production in skeletal muscle, which in turn is the result of abnormal Ca2+ leak from the sarcoplasmic reticulum (SR) and excessive production of oxidative species of oxygen and nitrogen. As a high fat diet is known to increase oxidative stress, the objective of the present study was to investigate the effects of 3 months of high-fat diet (HFD) on the HS susceptibility of wild type (WT) mice. HS susceptibility was tested in an environmental chamber where 4 months old WT mice were exposed to heat stress (41 °C for 1 h). In comparison with mice fed with a regular diet, mice fed with HFD showed: (a) increased body weight and accumulation of adipose tissue; (b) elevated oxidative stress in skeletal muscles; (c) increased heat generation and oxygen consumption during exposure to heat stress; and finally, (d) enhanced sensitivity to both temperature and caffeine of isolated muscles during in-vitro contracture test. These data (a) suggest that HFD predisposes WT mice to heat stress and (b) could have implications for guidelines regarding food intake during periods of intense environmental heat. |
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Keywords: | heat stroke high-fat diet malignant hyperthermia susceptibility skeletal muscle |
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