三七皂苷R1对铝佐剂甲型肝炎疫苗的免疫增强作用

目的探索三七皂苷R1对Al(OH)3佐剂甲型肝炎疫苗的免疫增强作用及降低铝佐剂用量的可行性。方法在甲型肝炎病毒(HAV)抗原中,加入不同剂量的铝佐剂与三七皂苷R1构成复合佐剂,经肌肉注射免疫小鼠,每隔4周检测小鼠血清HAV-IgG水平,并与无佐剂组和单独铝佐剂组进行比较。结果各免疫组抗体水平在12周时达到最高,之后逐渐下降。在抗原中加入复合佐剂后,与无佐剂组相比,IgG水平显著升高;与单独铝佐剂组相比,加入一定剂量的三七皂苷R1能够显著增强铝佐剂的作用,且抗体水平维持时间较长。结论适当剂量的铝佐剂与三七皂苷R1混合作为佐剂,具有增强铝佐剂的作用,并能降低铝佐剂的用量。

Sanchinoside R1 as An Immunopotentiator of Hepatitis A Vaccine Containing Aluminium Adjuvant

Objective To explore the effect of sanchinoside R1 as an immunopotentiator of hepatitis A(HA) vaccine containing aluminium adjuvant as well as the feasibility of decreasing the aluminium content in vaccine.Methods Add the compound adjuvant consisting of aluminium hydroxide and sanchinoside R1,at various dosages,to hepatitis A virus (HAV) antigen respectively.Inject i.m.mice with the prepared HA vaccines,determine the serum HAV-IgG every 4 weeks and compare with those of mice immunized by adjuvant-free vaccine and by the vaccine containing aluminium adjuvant.Results The HAV-IgG titers of mice in various test groups reached the peak values 12 weeks after immunization then decreased gradually.The IgG titer induced by vaccine containing compound adjuvant was significantly higher than that by adjuvant-free vaccine.The addition of sanchinoside R1 at a certain dosage enhanced the effect of adjuvant and prolonged the maintain time of antibody level.Conclusion The compound adjuvant consisting of aluminium hydroxide and sanchinoside R1 enhanced the immune effect of HA vaccine and decreased the dosage of aluminium adjuvant used.