| 大豆染色体端粒DNA的Southern及荧光原位杂交（FISH）分析 |
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| 引用本文： | 张德永 张志永. 大豆染色体端粒DNA的Southern及荧光原位杂交（FISH）分析[J]. 高技术通讯, 1998, 8(12): 7-10 |
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| 作者姓名： | 张德永 张志永 |
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| 作者单位： | 中国科学院遗传研究所 |
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| 摘 要： | 以拟南芥的端粒序列（Ｔ３ＡＧ３）ｎ为探针，经Ｂａｌ３１酶切检测和荧光原位杂交，证明端粒ＤＮＡ保守重复序列存在于大豆的全部２０对染色体端部，与拟南芥，番茄和人类不同，大豆全部２０对染色体端部上的这一序列的长度相似。
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| 关 键 词： | 大豆 端粒序列 Bal31分析 FISH 染色体 DNA |
Southern Blotting and FISH Analysis of Telomeric DNAs in Soybean (received July 3, 1998) |
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| Zhang Deshui,Zhang Zhiyong,Chen Shouyi. Southern Blotting and FISH Analysis of Telomeric DNAs in Soybean (received July 3, 1998)[J]. High Technology Letters, 1998, 8(12): 7-10 |
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| Authors: | Zhang Deshui Zhang Zhiyong Chen Shouyi |
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| Abstract: | It was reported the sequence of telomeric DNA in arabidopsis was (T 3AG 3)n. Using this sequence as a probe, Bal 31 digestion and fluoresent in situ hybridization(FISH) results showed that conserved repetitive telomeric DNA sequences existed in end regions of all the 20 pairs of soybean chromosomes. These telomeric sequences of soybean were found to be similar in length. This is quite different from arabidopsis, tomato and human. |
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| Keywords: | Soybean Telomeric sequence Bal 31 analysis FISH |
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