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| 16α，17α-环氧黄体酮C11β-羟基化微生物的选育 | |
| 引用本文： | 吴定伟,王丽娅,陈小龙,万建峰.16α，17α-环氧黄体酮C11β-羟基化微生物的选育[J].浙江化工,2011,42(10):9-12. |
| 作者姓名： | 吴定伟 王丽娅 陈小龙 万建峰 |
| 作者单位： | 1. 浙江仙琚制药股份有限公司,浙江仙居,317300 2. 浙江工业大学生物与环境工程学院,浙江杭州,310014 3. 浙江经贸职业技术学院应用工程系,浙江杭州,310018 |
| 摘 要： | 从S418黑根霉，蓝色犁头霉，蓝色犁头霉D1、D2、D5，新月弯孢霉X1、X2和雅致小克银汉霉8种菌株中筛选出能高效转化生产C11β-羟基-16α，17α-环氧孕酮的菌株-雅致小克银汉霉，并经低能氮离子注入诱变，获得一株性能稳定的银汉霉突变株，在28℃下摇瓶发酵22h，48h后投料，C11β-羟基-16α，17α-环氧孕酮的转化率达到5．8％，比最初的转化率提高30％。 |
| 关 键 词： | 甾体 C11β-羟化反应 雅致小克银汉霉 低能氮离子注入诱变 |
Breeding Microbial Strain for C11β-Hydroxylation of Steroid |
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| WU Ding-wei,WANG li-ya,CHEN Xiao-long,WAN Jian-feng.Breeding Microbial Strain for C11β-Hydroxylation of Steroid[J].Zhejiang Chemical Industry,2011,42(10):9-12. | |
| Authors: | WU Ding-wei WANG li-ya CHEN Xiao-long WAN Jian-feng |
| Affiliation: | 1.Zhejiang Xianju Pharmaceuticals Stock Co.Ltd.,Xianju 317300,China;2.College of Biological and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310014,China 3.Department of Applied Engineering,Zhejiang Economic & Trade Polytechnic,Hangzhou 310018,China) |
| Abstract: | To breed microbial strain for C11β--hydroxylation of steroid, Cunningamella elegans was selected as an original strain from eight strains for induced mutagenesis, which including Rhizopus nigricans, Absidia coerulea, A bsidia coerulea （D1, D2 and D5）, Curvularidunata （X1 and X2） and Cunningamella elegans. The original induced strain was treated by low-energy nitrogen ion implantation mutagenesis and one strain of highly positive mutation was obtained. With 22 h fermentation at 28℃, the conversion rate of C11β-hydroxylation reaching 5.8%, enhanced by 30% compared with the initial of 4.6%. |
| Keywords: | Steroid C11β-hydroxylation Cunninhamella elegans low-energy N ion implantation mutagenesis |
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