Definition of essential amino acid residues in the recognition of a peptide by a mouse monoclonal antibody |
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Authors: | L Rosanó F Di Modugno G Romagnoli A Chersi |
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Affiliation: | Istituto Regina Elena for Cancer Research, Laboratory of Biochemistry, Roma, Italy. |
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Abstract: | A mouse monoclonal antibody reacting in ELISA with a synthetic peptide representing a linear amino acid stretch of the protein antigen was tested on all overlapping 5-mer to 9-mer fragments of the peptide, as prepared by multi-pin synthesis. Analysis of the binding data suggests that several residues in the peptide might be relatively unrelevant for recognition, while few others seem to play a critical role as key residues. On the basis of such observations, we attempted to reconstruct an alternative essential epitope by introducing multiple amino acid substitutions in the 9-mer peptide exhibiting the best binding activity, and then tested its ability to be recognized by the monoclonal antibody. |
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