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| 非水溶剂电位滴定法测量碱基纯度 | |
| 引用本文： | 张玲,柳方方,陈大舟,王晶.非水溶剂电位滴定法测量碱基纯度[J].计量学报,2013,34(5):508-512. |
| 作者姓名： | 张玲 柳方方 陈大舟 王晶 |
| 作者单位： | 1. 中国计量科学研究院, 北京 100013; 2. 北京化工大学, 北京 100029 |
| 基金项目： | 国家科技支撑项目课题，中国计量科学院基本业务科研课题 |
| 摘 要： | 建立了在非水溶剂中电位滴定测定纯度标准物质胞嘧啶(Cyt)和腺嘌呤(Ade)含量的方法。通过实验对非水溶剂、滴定剂种类、滴定剂浓度、滴定终点进行了选择,确定了溶剂为冰醋酸HAc,滴定剂为高氯酸HClO4,滴定剂浓度为0.05 mo1／L,终点为电位曲线的等当点,实验用电位滴定曲线准确确定了滴定终点。通过15次测定,测得Ade的纯度为99.40%,不确定度度为0.38%(k=2);测得Cyt纯度为99.58%,不确定度0.42%(k=2)。 |
| 关 键 词： | 计量学 非水溶剂 电位滴定法 标准物质 |
Determine the Content of Nucleic Bases Cytosine and Adenine by Electrode Potential Titration in Non-aqueous Solvent |
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| ZHANG Ling , LIU Fang-fang , CHEN Da-zhou , WANG Jing.Determine the Content of Nucleic Bases Cytosine and Adenine by Electrode Potential Titration in Non-aqueous Solvent[J].Acta Metrologica Sinica,2013,34(5):508-512. | |
| Authors: | ZHANG Ling LIU Fang-fang CHEN Da-zhou WANG Jing |
| Affiliation: | 1. National Institute of Metrology, Beijing 100013, China; 2. Beijing University of Chemical Technology, Beijing 100029, China |
| Abstract: | A method to determine the content of nucleic bases Cytosine (Cyt) and Adenine (Ade) by electrode potential titration in non-aqueous soolvent is established.The factors affecting the experiments, such as non-aqueous solvent, titrant, the concentration of titrant, and end point of the titration are determined.The optimal experiment conditions determined are glacial acetic acid (HAc) as the non-aqueous solvent,perchloric acid as titrant (HClO4),titrant concentration 0.05 mol/L,and equivalence point of the electrode potential titration curve as endpoint.Through 15 times measurements and uncertainty evaluation, the purity of Ade is 99.40% ±0.38% (k=2), Cyt 99.58%±0.42% (k=2). |
| Keywords: | Metrology Non-aqueous solvent Electrode potential titration Certified reference material |
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