| 一种硅基集成PCR微芯片的传热数值分析 |
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| 引用本文： | 陈飞,邹志清,周洪波,金庆辉,赵建龙.一种硅基集成PCR微芯片的传热数值分析[J].功能材料与器件学报,2005,11(4):471-475. |
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| 作者姓名： | 陈飞 邹志清 周洪波 金庆辉 赵建龙 |
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| 作者单位： | 中国科学院上海微系统与信息技术研究所,生物芯片实验室,上海,200050;中国科学院研究生院,北京,100039;中国科学院上海微系统与信息技术研究所,生物芯片实验室,上海,200050;中国科学院上海微系统与信息技术研究所,生物芯片实验室,上海,200050;生物芯片上海国家工程研究中心,上海,201203 |
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| 基金项目： | 国家高技术“863计划”（No.2002AA222021）资助. |
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| 摘 要： | 设计和制作了一种基于MEMS技术的硅基集成PCR（聚合酶链式反应）微芯片，采用有限容积法，边界条件考虑自然对流换热和辐射换热，对PCR微芯片的传热过程进行了数值模拟。主要分析了样品的升、降温速度和样品内部的温度均一性。分析结果表明芯片具有较高的升、降温速度，而且样品内部的温度均一性也满足PCR反应的要求。芯片在温度控制系统下进行了热循环反应，实现了GUS基因的扩增，获得了良好的实验结果。
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| 关 键 词： | PCR微芯片 传热 数值模拟 |
| 文章编号： | 1007-4252（2005）04-0471-05 |
| 收稿时间： | 2005-03-14 |
| 修稿时间： | 2005-04-30 |
Numerical simulation of heat transfer for an integrated PCR microchip |
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| CHEN Fei,ZOU Zhi-qing,ZHOU Hong-bo,JIN Qing-hui,ZHAO Jian-long.Numerical simulation of heat transfer for an integrated PCR microchip[J].Journal of Functional Materials and Devices,2005,11(4):471-475. |
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| Authors: | CHEN Fei ZOU Zhi-qing ZHOU Hong-bo JIN Qing-hui ZHAO Jian-long |
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| Affiliation: | 1. Biochip Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China;2. Graduate School of the Chinese Academy of Sciences, Beijing 100039,China; 3. National Engineering Center for Bioehip at Shanghai, Shanghai 201203, China |
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| Abstract: | A PCR(Polymerase Chain Reaction) microchip was designed and fabricated on a silicon wafer.The temperature characteristics of the chip were simulated using CFD-ACE~+.Natural convective and radiative heat transfer were taken into account as the boundary conditions.The simulation was mainly used to analyze the temperature distribution of the sample and the rate of heating and cooling.The analysis results prove that the chip has higher temperature rising and cooling rates,and temperature distribution of the sample can meet the requirement of the PCR.By combining peripheral thermal control system,the GUS gene is successfully amplified in chip. |
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| Keywords: | PCR microchip heat transfer numerical simulation |
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