黑果腺花楸不同极性物质抗氧化及抗炎镇痛研究

为了高效的提取及分离黑果腺花楸活性物质,以黑果腺花楸为原料,不同极性溶剂作为溶媒(石油醚、乙酸乙酯、正丁醇和水),采用超声波辅助提取法,分别提取到不同极性物质(石油醚相、乙酸乙酯相、正丁醇相和水相)。利用不同的抗氧化模型(铁还原力、清除羟基自由基(OH)·能力、清除ABTS自由基能力、抗肝组织自发性脂质过氧化能力和花生油氧化能力)、炎症模型(小鼠耳廓肿胀和小鼠肉芽肿胀)和镇痛模型(小鼠热板痛阈值和冰醋酸致小鼠扭体次数)来评价黑果腺花楸不同极性物质的活性。结果表明,正丁醇相具有较强的抗氧化能力和消炎镇痛能力。正丁醇可以作为提取黑果腺花楸活性物质及分离抗氧化活性及抗炎镇痛成分的溶剂。

Study on Antioxidant,Anti-inflammatory and Analgesic Activity of Different Polar Parts from Aronia Melanocarpa

In order to efficiently extract and separate the active components from Aronia melanocarpa,taking Aronia melanocarpa as raw material,different polar parts(petroleum ether phase,ethyl acetate phase,n-butanol phase and water phase)were extracted with different polar solvents(petroleum ether,ethyl acetate,n-butanol and water)by using ultrasonic wave assisted extraction method.Different antioxidant models(icon-reducing ability,hydroxyl radical scavanging capacity,ABTS radical scavenging activity,inhibitory effects on lipid peroxidation),inflammatory models(mouse ear swelling and mouse granuloma)and analgesic models(heat sensitivity in mice and the number of writhing responses induced by acetic acid in mice)were used to evaluate biological acitivity of different polar parts.The results showed that n-butanol phase has better antioxidant capacity,anti-inflammatory and analgesic capacity.n-Butanol could be used as a solvent for extracting active substances and separating antioxidant,anti-inflammatory and analgesic components from Aronia melanocarpa.