酒糟总氨基酸、总多酚、多糖含量测定及抗氧化活性研究

酿酒行业每年都会产生大量酒糟,为减少酒糟的丢弃浪费与环境污染,研究酒糟内总成分含量、总抗氧化能力,可为后期开发利用提供理论支持。研究采用茚三酮法、福林酚法、苯酚-硫酸法测定酒糟内总氨基酸、总多酚、多糖含量,同时采用1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-trinitrophenylhydrazyl,DPPH)法、2,2′-联氮双-(3-乙基苯并噻唑林-6-磺酸)二胺盐2,2′-Azinobis(3-ethylbenzothi azoline-6-sulfonic acid)ammonium salt,ABTS]法、超氧阴离子(Superoxide anion,O^-₂)法测定酒糟液不同稀释倍数抗氧化能力。谷物酿酒后的副产物中总氨基酸、总多酚、多糖平均含量分别达到9.7998、0.6289、2.8203 mg/mL,对DPPH、ABTS、O^-₂的自由基清除率均达到90%以上。酒糟液富含大量氨基酸、多酚、多糖,有较强抗氧化能力,可开发为新型产品,为企业带来下游效益的同时减轻环境污染,为后续高效利用酒糟液奠定基础。

Determination of Total Amino Acids,Total Polyphenols,Polysaccharide Content and Antioxidant Activity of Distiller′s Grains

Liquor-making industry produces a large amount of distiller′s grains every year.In order to reduce the waste and environmental pollution of distiller′s grains,the total composition content and total antioxidant capacity of distiller′s grains were studied to provide theoretical support for later development and utilization.In this study,the contents of total amino acids,total polyphenols and polysaccharides in distiller′s grains were determined by ninhydrin method,folin-ciocalteu method and phenol-sulfuric acid method.At the same time,1,1-diphenyl-2-trinitrophenylhydrazyl(DPPH)method,2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt(ABTS)method,superoxide anion(O^-₂)method were used to determine the antioxidant capacity of different dilution multiples of distiller′s grains.The average contents of total amino acids,total polyphenols and polysaccharides in the by-products after liquor-making reached 9.7998 mg/mL,0.6289 mg/mL and 2.8203 mg/mL,respectively.The free radical scavenging rates of DPPH,ABTS and O^-₂ reached more than 90%.Distillers′grains liquid is rich in a large number of amino acids,polyphenols and polysaccharides,and has strong antioxidant capacity.It can be developed as a new product,which will not only bring downstream benefits to enterprises,but also reduce environmental pollution and lay a foundation for the efficient utilization of distillers′grains in the future.