草果不同极性萃取物总黄酮、总多酚含量与其抗氧化活性的相关性

以草果为原料,通过50%乙醇、水、乙酸乙酯、丙酮、正丁醇、甲醇和石油醚不同极性溶剂萃取活性物质,探讨萃取物中总黄酮和总多酚含量与总还原能力及对DPPH自由基清除率、ABTS⁺自由基清除率、OH^-自由基清除率、NO^-₂清除能力的相关性。结果表明:50%乙醇萃取物总黄酮和总多酚含量均最高,各萃取物均有抗氧化能力,且呈明显的量效关系,其中50%乙醇萃取物总还原能力、清除ABTS⁺和DPPH能力最强,石油醚萃取物清除OH^-和NO^-₂能力最强。相关性分析表明,草果萃取物中总黄酮含量与ABTS⁺清除能力呈极显著相关,与DPPH清除能力呈显著相关,总多酚含量与ABTS⁺和DPPH的清除能力呈极显著相关,可为草果作为天然抗氧化剂提供理论依据。

Correlation between the Content Total Flavonoids and Total Polyphenols in Amomum Tsaoko with Different Polar Extracts and Their Antioxidant Activities

The active materials were extracted with 50%ethanol,water,ethyl acetate,acetone,n-butanol,methanol and petroleum ether from Amomum tsaoko.The correlation of the contents of total flavonoids,total polyphenols with the total reducing ability and the scavenging ability of DPPH radical scavenging,ABTS⁺ radical scavenging,OH^-radical scavenging and NO^-₂ were explored.The results showed that the highest total flavonoids and total polyphenols contents were obtained in 50%ethanol extract.All extracts from Amomum tsaoko had antioxidant activity and showed a dose effect relationship.The 50%ethanol extract from Amomum tsaoko had the strongest scavenging ability of DPPH and ABTS⁺ and the best reducing power.The petroleum ether extract had the strongest scavenging ability of OH^- and NO^-₂.Correlation analysis showed that the total flavonoids contents were significantly correlated with the scavenging ability of DPPH and extremely significant correlation with the scavenging ability of ABTS⁺.The total polyphenols contents were extremely significant correlation with the scavenging ability of ABTS⁺ and DPPH.The results could provide a theoretical basis for the development of Amomum tsaoko as a natural antioxidant.