玉米黄粉酶解液在黄原胶发酵生产应用的研究

以玉米黄粉酶解液为氮源应用于黄原胶发酵培养基，探讨了蛋白酶种类、玉米黄粉酶解液添加量和酶添加量等因素对其发酵黄原胶粘度和产胶率的影响，并优化了复配氮源的种类及其复配比例。结果表明，碱性蛋白酶水解液作为氮源发酵黄原胶的粘度和产胶率优于其它蛋白酶解液；当酶解液添加量为6%、碱性蛋白酶添加量为8kU时，发酵黄原胶的粘度和产胶率可以达到4235cp和24.4g/L。大豆蛋白与玉米黄粉酶解液复配效果优于与棉籽粉、豆粕粉、花生粉的复配；玉米黄粉酶解液与大豆蛋白复配比为3:7时，发酵黄原胶的粘度和产胶率可以达到5833cp和32.4g/L，与单独以大豆蛋白为氮源无明显差异。本研究为提高玉米黄粉资源利用率、降低黄原胶的发酵生产成本提供了新思路。

The Application of Corn Gluten Meal hydrolysate in Xanthan Fermentation

To apply enzymatic hydrolysate of corn gluten meal (CGM) as nitrogen source to xanthan fermentation, we discussed the effect of protease type, enzymatic hydrolysate dosage and enzyme dosage on productive rate and viscosity of xanthan. Then, the optimal nitrogen source combined with CGM hydrolysate and the ratio of CGM hydrolysate to soybean protein were optimized by single factor experiment. The results indicated that alkaline protease pretreatment had a better effect on the following xanthan fermentation than other proteases. Productive rate and viscosity of xanthan were 24.4g/kg and 4235cp when CGM dosage was 6% and enzyme dosage was 8kU. The single factor experiments showed that soybean protein combined with CGM hydrolysate as nitrogen source was better than other proteins. Furthermore, the optimal ratio of CGM hydrolysate to soybean protein was 4:6. Under above conditions, productive rate and viscosity of xanthan could reach 32.4g/kg and 5833cp, which were consistent with the value of soybean protein as the sole nitrogen source. This study provides a new idea for the reuse of corn gluten meal and a possible low-cost approach for xanthan fermentation.