Development of an image system for the study of viable but non-culturable forms ofCampylobacter jejuniand its use to determine their resistance to disinfectants |
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| Affiliation: | 1. CSIRO Oceans and Atmosphere Flagship, Canberra, Australia;2. CSIRO Oceans and Atmosphere Flagship, Hobart, Australia;3. Coastal and Marine Unit, Office of Environment and Heritage, NSW Department of Premier and Cabinet, Sydney, Australia;4. Italian National Research Council (CNR) — Institute for Electromagnetic Sensing of the Environment (IREA), Milan, Italy;5. Plant Functional Biology and Climate Change Cluster, University of Technology, Sydney, Australia;1. Kanazawa University, Kakuma, Kanazawa, Ishikawa 920-1156, Japan;2. Asia Center for Air Pollution Research, 1182, Sowa, Niigata, Niigata 950-2144, Japan;3. Niigata Prefectural Institute of Public Health and Environmental Sciences, 314-1, Sowa, Niigata, Niigata 950-2144, Japan;4. Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan;5. Japan Environmental Sanitation Center, 10-6 Yotsuyakami-cho, Kawasaki-ku, Kawasaki City, Kanagawa Prefecture 210-0828, Japan;6. National Institute of Advanced Industrial Science and Technology, 16-1 Onogawa, Tsukuba 305-8569, Japan;7. Shimane Prefectural Institute of Public Health and Environmental Sciences, 1-582, Nishimasasada cho, Matsue, Shimane 690-0122, Japan;8. Okinawa Prefectural Institute of Health and Environment, 1-17, Kanekadann, Uruma, Okinawa 904-2241, Japan;9. Nagano Environmental Conservation Research Institute, 1978 Komemura Amori Nagano, Nagano 380-0944, Japan;10. Hokkaido Research Organization, 12-19, Nishi, Kitaku, Sapporo, Hokkaido 060-0819, Japan |
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| Abstract: | A vital staining method incorporating fluorescence microscopy and image analysis has been developed to study the viable but non-culturable (VNC) form ofCampylobacter jejuni. This method was used to determine the comparative resistance of the culturable and VNC forms to proprietary disinfectants chosen to represent the four main groups of disinfectants used in food processing operations, namely quaternary ammonium compounds (QACs) and QACs combined with glutaraldehyde, amphoterics and chlorine. With all disinfectants except chlorine the culturable form was significantly more resistant than the VNC form. The resistance of the VNC form to chlorine may have significance for cleaning and decontamination in the food industry. |
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