牛血清蛋白在亲水硅片表面吸附的原子力显微成像

本文用原子力显微术（AFM）研究了牛血清白蛋白（BSA）在亲水硅片表面的吸附，硅片表面经亲水处理后，将牛血清蛋白（BSA）吸附在表面，采用轻敲模式，可获得清晰的AFM图像，牛血清蛋白（BSA）的AFM图像表明：BSA在亲水硅片表面是单分子，水平吸附在硅片表面，且吸颗粒状；1mg/ml的BSA在吸附30min后为饱和吸附。BSA到达硅表面后，蛋白中可移动的带正电荷的基团可以趋向亲水表面，使BSA与硅表面的静电相互作用由斥力变为吸引力，BSA可以稳定地吸附在亲水硅片表面。

Study of the adsorption of bovine serum albumin on hydrophilic silicon surface by atomic force microscope

Atomic force microscope was used to study the adsorption of bovine serum albumin on hydrophilic silicon surface. The samples were imaged with an AutoProbe CP Research Scanning Probe Microscope (Park Scientific Instruments) with IC AFM mode. These AFM images showed that bovine serum albumin adsorbed flatly in grain shape and mono molecules. The adsorption of bovine serum albumin reached its saturation after 30min (1mg/ml). When bovine serum albumin arrived at surface, the molecules might rearrange to bring the most mobile regions of positive charge close to the interface, so BSA could be adsorbed on the hydrophilic surface steadily.