壳聚糖/羧甲基壳聚糖止血膜的制备与表征

针对壳聚糖止血膜柔韧性差的问题,以丙三醇和酚磺乙胺作为改性剂,采用溶液浇膜的方法制备了不同组成配比的壳聚糖(CS)/羧甲基壳聚糖(CMCS)止血膜,并评价了CS/CMCS止血膜拉伸性能和止血效果.丙三醇的引入可使CS/CMCS止血膜的断裂伸长率提高4~7倍,尽管其拉伸强度也降低了70%.丙三醇改性的CS/CMCS=8/2载药止血膜具有较好的拉伸强度(10.4±0.9 MPa)和断裂伸长率(50.4±4.5%);与未加入酚磺乙胺的CS/CMCS止血膜相比,酚磺乙胺的离子交联作用使CS/CMCS载药止血膜的拉伸强度提高55%,而断裂伸长率仅降低24%.止血评价结果表明经丙三醇改性的CS/CMCS=8/2止血膜均能使血液发生分离的时间明显缩短,并具有促使血液凝结形成黑色血块的能力.

Preparation and characterization of chitosan/carboxymethyl chitosan hemostatic membrane

Aimed at the poor toughness of chitosan hemostatic membrane,glycerol and etamsylate were chosen as modification reagents to prepare chitosan(CS)/carboxymethyl chitosan(CMCS) hemostatic membrane with different weight ratios by solution casting method.The effects of glycerol and etamsylate on mechanical properties and hemostatic efficiency were evaluated by tensile tests.The blood coagulation time was characterized to illustrate the interactions between CS/CMCS hemostatic membrane and blood.Results indicate that the elongation at break of CS/CMCS hemostatic membrane modified by glycerol without etamsylate is increased by 4～7 times,although the tensile strength of that is decreased by 70%.The tensile strength and the elongation at break of CS/CMCS hemostatic membrane modified by glycerol and etamsylate are 10.4±0.9MPa and 50.4±4.5%,respectively.Compared with the CS/CMCS hemostatic membrane modified by glycerol without etamsylate,the tensile strength is increased by 55% and the elongation at break is merely decreased by about 24% attributed to the ions crosslinking of sulfonate.Results of the hemostatic test show that CS/CMCS=8/2 hemostatic membrane modified by glycerol can accelerate the blood separation and induce the blood coagulation to form black clotting.