Electrospun poly (N-isopropylacrylamide-co-acrylic acid)/cellulose laurate blend nanofibers containing adapalene: Morphology,drug release,and cell culture studies

In this work, a thermo- and pH-sensitive polymer based on poly (N-isopropylacrylamide-co-acrylic acid)/cellulose laurate blend [P(NIPAAm-co-AAc)/CL] was electrospun to produce nanofibers containing adapalene. The synthesized P(NIPAAm-co-AAc), and CL were characterized by ATR-FTIR and ¹H NMR spectroscopies. The thermal behavior of the electrospun nanofibrous samples were determined by a DSC. Afterward, the drug release in different temperatures and pHs from the nanofibers was investigated by UV-vis spectrophotometry and then the LCST behavior of the samples containing adapalene was observed by an ESEM. Moreover, the fibroblast cells culture was carried out with and without adapalene and the cell cytotoxicity of the samples was evaluated via MTT assay. The results from (H&E) and DAPI staining of the viable cells onto the samples surfaces revealed the adapalene has a remarkable short-term potential for the fibroblast cells mortality. It was found that the adapalene loaded electrospun nanofibrous samples enabled to minimize the adverse side effects of the drug by controlling release during three days of cell culture.