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The odontogenic differentiation of human dental pulp stem cells on hydroxyapatite-coated biodegradable nanofibrous scaffolds |
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| Authors: | Fatemeh Asghari Roya Salehi Marziyeh Agazadeh Effat Alizadeh Khosro Adibkia Mohammad Samiei |
| Affiliation: | 1. School of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran;2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;5. School of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran;6. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;7. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;8. School of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran |
| Abstract: | The authors aimed to design nanofibrous (NF) scaffolds that facilitate odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (DPSCs) in vitro. For this purpose, hydroxyapatite (HA)–loaded poly (L-lactic acid)/poly (?-caprolactone) (PLLA:PCL 2;1) blend NFs were prepared using the electrospinning method. Alizarin red activity and cell viability were evaluated by MTT assay, and SEM revealed the proliferation properties of NF scaffolds. QRT-PCR results demonstrated that HA-loaded PLLA/PCL can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that electrospun biodegradable PCL/PLA/HA has remarkable prospects as scaffolds for bone and tooth tissue engineering. |
| Keywords: | Dental pulp stem cells differentiation nanofibers odontogenic regenerative medicine scaffold tissue engineering |