MicroSPE-nanoLC-ESI-MS/MS using 10-microm-i.d. silica-based monolithic columns for proteomics |
| |
Authors: | Luo Quanzhou Page Jason S Tang Keqi Smith Richard D |
| |
Affiliation: | Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. |
| |
Abstract: | Silica-based monolithic capillary columns (25 cm x 10 microm i.d.) with integrated nanoESI emitters have been developed to provide high-quality and robust microSPE-nanoLC-ESI-MS analyses. The integrated nanoESI emitter adds no dead volume to the LC separation, allowing stable electrospray operation at flow rates of approximately 10 nL/min. In an initial application with a linear ion trap MS, we identified 5510 unique peptides that covered 1443 distinct Shewanella oneidensis proteins from a 300-ng tryptic digest sample in a single 4-h LC-MS/MS analysis. The use of an integrated monolithic ESI emitter provided enhanced resistance to clogging and provided good run-to-run reproducibility. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|