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Specific integrin labeling in living cells using functionalized nanocrystals |
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| Authors: | Lieleg Oliver López-García Mónica Semmrich Christine Auernheimer Jörg Kessler Horst Bausch Andreas R |
| Affiliation: | 1. Lehrstuhl für Biophysik E22, Technische Universit?t München, James‐Franck‐Strasse 1, 85748 Garching, Germany, Fax: (+49)?89‐289‐12469;2. Lehrstuhl für Organische Chemie II, Technische Universit?t München, Lichtenbergstrasse 4, 85748 Garching, Germany;3. Klinikum rechts der Isar der Technischen Universit?t München, Nuklearmedizinische Klinik und Poliklinik, Ismaningerstrasse 22, 81675 München, Germany |
| Abstract: | We present an integrin labeling method using functionalized quantum dots (QDs). Cyclic Arg-Gly-Asp (RGD) peptides and a biotin-streptavidin linkage are used to specifically couple individual QDs to integrins of living cells. The spacer distance between the RGD sequence and the QD surface is a crucial parameter to ensure specific binding to individual alpha(v)beta(3) integrins of osteoblast cells. Despite blinking, the position of single QDs is tracked with nanometer precision and localized diffusive behavior is observed. We show that blinking events do not prevent the acquisition of quantitative parameters from the QD trajectories. |
| Keywords: | bioimaging cells labeling peptides quantum dots |
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