高压液相色谱法同时测定食品中的维生素A、D、E和K

本方法研究了应用反相高压液相色谱技术,同时测定食品中的维生素A、D、E、K。以C18柱为固定相(μ Bondapak C18,30cm×4mm),甲醇作流动相。在290nm下测定维生素A、E,在265nm下测定维生素D、K。样品的前处理分为两种,第一种皂化法,可同时测定维生素A、D、E。加标样品的回收率分别为:V_A97.2～110％,V_D92.3～102％,V_E96.6～110％。变异系数(CV％)分别为V_A2.29～3.15％,VD5.99～8.63％,V_E3.60～4.16％。第二种为酶解法,可同时测定维生素A、D、E、K四种,加标样品的回收率分别为:V_A87.62～98.01％,V_D90.23～102.7％,V_E86.18～91.09％,V_K80.15～85.07％。变异系数(CV％)分别为:V_A5.51％,V_D8.87％,V_E4.91％,V_K3.67％。

Simultaneous Determination of Vitamin A, D, E and K in Foods by HPLC

A reversed phase high performance liquid chromatogrphic method for the simultaneous determination of vitamins A, D, E and K in foods has been developed. Separation is achieved using a μ.Bondapak C18 column (30cm×4mm), A mobile phase is methanol. Vitamin A,E are detected at 290 nm, and vitamin D,K are detected at 265 nm. Sample perparation have two methods, first one is saponification, the recoveries of vitamin A,D,E by simultaneous determination is 97.2-110%, 92.3 -102%, 96.6-110% respectively, the coefficients of variation is 2.29-3.15%, 5.99-8.63%, 3.60-4.14% respectively. Second method requires enzymatic hydrolysis of the sample, the recoveries of vitamin A,D,E and K by simultaneous determination is 87.62-98.01%, 90.23-102.7%, 86.18-91.09%, 80.15-85.07% respectively, the coefficients of variation is 5.51%, 8.87%, 4.91%, 3.67% respectively.