| 胰岛素免疫放射分析方法的建立 |
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| 引用本文： | 董宏广,贾娟娟,刘一兵,许文革. 胰岛素免疫放射分析方法的建立[J]. 同位素, 2007, 20(3): 167-171. DOI: 10.7538/tws.2007.20.03.0167 |
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| 作者姓名： | 董宏广 贾娟娟 刘一兵 许文革 |
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| 作者单位： | 中国原子能科学研究院同位素研究所 北京102413 |
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| 摘 要： | 摘要：采用两株抗胰岛素单克隆抗体，一株包被聚苯乙烯试管作为固相抗体、另一株标记125I，建立了胰岛素测定的双抗体夹心免疫放射分析方法。其方法学灵敏度为2.8mIU/L；曲线范围5～160mIU/L；批内、批间变异系数分别小于5%、10%；回收率92.3％～112.6％；高浓度胰岛素血清样品系列倍比稀释后测定，测定值与稀释度呈线性相关，相关系数0.996；39例健康人血清样品测定值为4.7～14.3mIU/L，该方法与胰岛素放射免疫分析法的相关性良好（r=0.981）。
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| 关 键 词： | 胰岛素 单克隆抗体 免疫放射分析 |
| 文章编号： | 1000-7512（2007）03-0167-04 |
| 收稿时间： | 2007-01-09 |
| 修稿时间： | 2007-05-30 |
Establishment of Immunoradiometric Assay for Insulin |
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| DONG Hong-guang,JIA Juan-juan,LIU Yi-bing,XU Wen-ge. Establishment of Immunoradiometric Assay for Insulin[J]. Isotopes, 2007, 20(3): 167-171. DOI: 10.7538/tws.2007.20.03.0167 |
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| Authors: | DONG Hong-guang JIA Juan-juan LIU Yi-bing XU Wen-ge |
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| Affiliation: | Department of Isotope, China Institute of Atomic Energy, Beijing 102413,China |
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| Abstract: | Two monoclonal antibodies against insulin are used,one of the antibodies is immobilized to plastic tubes as the solid phase,the other is labelled with 125I as a tracer.The sensitivity of the assay is 2.8 mIU/L and the recovery is 92.3%-112.6%.The intraassay and interassay CVs are below 5% and 10% respectively.The correlation coefficient between measured and expected values is 0.996 after serial dilutions of the samples with high concentration of insulin.The value for normal samples(n=39) is 4.7-14.3 mIU/L.Compared with RIA,the correlation is acceptable(r=0.981). |
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| Keywords: | insulin monoclonal antibody immunoradiometric assay |
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