| 乳清蛋白酶解制备ACE抑制肽的研究 |
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| 引用本文： | 李朝慧,罗永康,王全宇.乳清蛋白酶解制备ACE抑制肽的研究[J].中国乳品工业,2005,33(2):8-11. |
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| 作者姓名： | 李朝慧 罗永康 王全宇 |
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| 作者单位： | 1. 中国农业大学,食品科学与营养工程学院,北京,100083
2. 北京银河奶业技术开发公司,北京,100029 |
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| 基金项目： | 国家“十五”攻关项目(2002BA518A10) |
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| 摘 要： | 采用碱性蛋白酶、中性蛋白酶、胃蛋白酶、胰蛋白酶和木瓜蛋白酶水解乳清蛋白制备ACE抑制肽，通过体外检测法测定其ACE抑制率。结果表明，碱性蛋白酶水解物的ACE抑制率最大。采用三因素二次通用旋转设计对碱性蛋白酶水解乳清蛋白的水解条件进行优化。研究了底物浓度、温度和酶与底物的质量比对ACE抑制率的影响，建立了回归方程，分析了各因素对ACE抑制率的影响．确定了最优的水解条件。
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| 关 键 词： | 乳清蛋白 ACE抑制肽 蛋白酶 水解物 |
| 文章编号： | 1001-2230(2005)02-0008-04 |
Preparation of angiotensin I converting enzyme inhibition from whey protein |
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| LI Zhao-hui,LUO Yong-kang,WANG Quan-yu.Preparation of angiotensin I converting enzyme inhibition from whey protein[J].China Dairy Industry,2005,33(2):8-11. |
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| Authors: | LI Zhao-hui LUO Yong-kang WANG Quan-yu |
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| Affiliation: | LI Zhao-hui1,LUO Yong-kang1,WANG Quan-yu2 |
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| Abstract: | ACE inhibitory peptides were prepared from whey protein by five commercial proteases(alcalase, neutrase, pepsin, trypsin, papain), and their ACE inhibitory activity were determined in vitro by spectrophotometry assay. The alcalase hydrolysates was found to have the highest ACE inhibitor activity. The effects of protein concentration, hydrolysis temperature and enzyme to substrate ratio on ACE inhibitor activity were studied by orthogonal rotational regression design.The regression equation of ACE inhibitory activity with the protein concentration, hydrolysis temperature and enzyme to substrate ratio was established. |
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| Keywords: | whey protein ACE inhibitory peptide proteinase hydrolysates |
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