| 重组人白细胞介素-6包含体溶解工艺研究 |
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| 引用本文： | 吴蕾,张瑛,甘一如,孙江莉,张浩森.重组人白细胞介素-6包含体溶解工艺研究[J].化学工业与工程,2000,17(6):321-325. |
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| 作者姓名： | 吴蕾 张瑛 甘一如 孙江莉 张浩森 |
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| 作者单位： | 天津大学化工学院!天津300072,天津大学化工学院!天津300072,天津大学化工学院!天津300072,天津大学化工学院!天津300072,天津大学化工学院!天津300072 |
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| 基金项目： | 国家科技攻关项目，96-C02-01-04， |
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| 摘 要： | 本文系统研究了影响大肠杆菌表达的重组人白细胞介素 6 (rhIL 6 )包含体溶解的因素 ,确定了rhIL 6包含体溶解的最佳工艺条件 ,即在 pH为 8 4的 5 0mmol/LTris HCL缓冲体系中 ,变性剂尿素和还原剂 β 基乙醇 (β ME)的浓度分别为 8mol/L和 5 0mol/L ,包含体含量为 1 0mg/mL ,在 4℃下溶解 1h。包含体溶解后蛋白含量可达到 1 3mg/mL左右 ,目标蛋白的溶解率达到 98% ,纯度为 5 0 %左右
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| 关 键 词： | 重组人白细胞介素-6 包含体 |
| 修稿时间： | 1999-07-18 |
A Study for Solubilization of rhIL-6 Inclusion Body Produced from E.Coli |
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| WU Lei,ZHANG Ying,GAN Yi-ru,SUN Jiang-li,ZHANG Hao-sen.A Study for Solubilization of rhIL-6 Inclusion Body Produced from E.Coli[J].Chemical Industry and Engineering,2000,17(6):321-325. |
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| Authors: | WU Lei ZHANG Ying GAN Yi-ru SUN Jiang-li ZHANG Hao-sen |
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| Affiliation: | (School of Chemical Engineering,Tianjin University,Tianjin 300072,China |
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| Abstract: | The technological condition for solubilization of the recombinant human Interleuk in\|6(rhIL 6) inclusion body produced from E.coli was studied. The optimal solubilization condition was obtained:the inclusion body was solubilized at 4℃ in 50mmol/L Tris\|HCL(pH=8.4),8mol/L urea and 50 mM β ME at a concentration of 10mg/mL for about 1h. Then nearly all the target protein was solubilized and the solubilized protein concentration was solubilized and the solubilized protein concentration was about 1.3 mg/mL and the purity was up to 50%. |
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| Keywords: | Recombinat human Interleukin-6 (rhIL-6) Inclusion body Solubilization |
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