| 纳米磁性微球对人血浆抗体的特异性分离过程 |
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| 引用本文： | 王胜男,张莹雪,林志勇,钱浩. 纳米磁性微球对人血浆抗体的特异性分离过程[J]. 材料导报, 2011, 25(16) |
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| 作者姓名： | 王胜男 张莹雪 林志勇 钱浩 |
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| 作者单位： | 1. 华侨大学材料科学与工程学院,厦门,361021
2. 华侨大学信息科学与工程学院,厦门,361021 |
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| 基金项目： | 国家自然科学基金(5060300720906035); 福建省高等学校新世纪优秀人才支持计划(07FJRC02) |
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| 摘 要： | 采用细乳液聚合法制备了嗜硫磁性纳米微球,磁球粒径较小,具有灵敏的磁响应性。经嗜硫性配基(2-巯基噻唑啉)修饰后,在pH=4.0的低浓度柠檬酸缓冲液中可特异性吸附免疫球蛋白G,并实现了在较低盐浓度下被吸附抗体的有效脱附,在吸附过程中表现出明显的单分子层吸附过程。在分离过程中几乎不损伤抗体的生物活性,所分离抗体的生物活性高于99%。磁性微球的平衡吸附量为53.04mg/g,相比于微米级磁性微球的平衡吸附量(3.1mg/g),提高了17倍。
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| 关 键 词： | 细乳液聚合 嗜硫纳米微球 2-巯基噻唑啉 免疫球蛋白G |
Specific Isolation of Immunoglobulin G from Human Plasma with Magnetic Nanobeads |
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| WANG Shengnan,ZHANG Yingxue,LIN Zhiyong,QIAN Hao. Specific Isolation of Immunoglobulin G from Human Plasma with Magnetic Nanobeads[J]. Materials Review, 2011, 25(16) |
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| Authors: | WANG Shengnan ZHANG Yingxue LIN Zhiyong QIAN Hao |
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| Affiliation: | WANG Shengnan1,ZHANG Yingxue2,LIN Zhiyong1,QIAN Hao1 (1 College of Materials Science and Engineering,Huaqiao University,Xiamen 362021,2 College of InformationScience and Engineering,Xiamen 362021) |
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| Abstract: | Nano-sized magnetic polymer spheres with intense magnetic response were synthesized by miniemulsion polymerization.After modified by 2-mercaptothiazoline on the surface of these magnetic nanospheres,these magnetic particles exhibited a strong specificity towards IgG.It was found that the qe(53.04mg/g) of magnetic polymer nanospheres increased by 17 times compared with the qmax(3.1mg/g) of micro-spheres.This specific binding of IgG could be performed at low citric acid concentration(pH=4.0) and the respondin... |
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| Keywords: | miniemulsion polymerization thiophilic magnetic nanospheres 2-mercaptothiazoline IgG |
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