Enzyme-assisted aqueous extraction of peanut oil |
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Authors: | Aparna Sharma S. K. Khare M. N. Gupta |
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Affiliation: | (1) Chemistry Department, Indian Institute of Technology, Hauz Khas, 110 016 New Delhi, India |
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Abstract: | Enzyme-assisted aqueous extraction of oil from oilseeds is a relatively recent technique. In the present study, peanut oil was extracted under optimized aqueous extraction conditions using Protizyme™, which is predominantly a mixture of acid, neutral, and alkaline proteases. The optimal conditions were: enzyme concentration of 2.5% (w/w) in 10 g of peanut seeds, pH 4.0, 40°C, and 18 h incubation with constant shaking at 80 rpm. Centrifuging the mixture at 18,000 × g for 20 min separated the oil with a recovery of 86–92%. The merits of this process over existing solvent extraction and/or mechanical pressing methods are discussed. |
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Keywords: | Enzyme-assisted aqueous extraction of oil peanut oil |
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