| 重组鲈鱼生长激素的分离纯化及抗体制备 |
| |
| 引用本文： | 曾润颖,刘小青,杨丰. 重组鲈鱼生长激素的分离纯化及抗体制备[J]. 高技术通讯, 2001, 11(5): 13-16 |
| |
| 作者姓名： | 曾润颖 刘小青 杨丰 |
| |
| 作者单位： | 国家海洋局第三海洋研究所海洋生物工程重点实验室厦门 361005 |
| |
| 基金项目： | 国家“九五”攻关课题 ( 96 C0 1 0 5 0 4)，福建省科技资助项目 ( 99 Z 9) |
| |
| 摘 要： | 用IPTG诱导鲈鱼生长激素基因工程菌E.coli.RV308，使重组鲈鱼生长激素得以表达并分泌到周质空间，利用渗透休克法从菌液中提取间质蛋白，经DEAE-SepharoseCL-6B、矣丙烯酰胺凝胶电泳及SephadexG-75纯化后得到单一电泳纯的重组鲈鱼生长激素，并将此激素作为抗原制备抗体，可应用于鱼类血液中生长激素含量的测定。
|
| 关 键 词： | 重组鲈鱼生长激素 纯化 抗体 分离 制备 |
Isolation and Purification of Recombinant Fish Growth Hormone (RFGH) and Preparation of Antibody Against the RFGH |
| |
| Zeng Runying,Liu Xiaoqing,Yang Feng. Isolation and Purification of Recombinant Fish Growth Hormone (RFGH) and Preparation of Antibody Against the RFGH[J]. High Technology Letters, 2001, 11(5): 13-16 |
| |
| Authors: | Zeng Runying Liu Xiaoqing Yang Feng |
| |
| Abstract: | The Fish Growth Hormone gene of Japanese sea perch ( Lateolabrax japonicus, jsp ) was cloned into the E coli. RV308 In the presence of IPTG, the recombinant Fish Growth Hormone (rFGH) was expressed and secreted into periplasmic space By osmotic shock method, recombinant proteins were extracted and then purified utilizing DEAE Sepharose CL 6B, native PAGE and Sephadex G 75 in turn The result of SDS PAGE showed that single protein was obtained Specific rFGH antiserum was prepared in mouse with purified rFGH. |
| |
| Keywords: | Recombinant japanese sea perch fish growth hormone Purification Antiserum |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
