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| 应用基因芯片技术检测食品中金黄色葡萄球菌 | |
| 引用本文： | 何洋,周黎黎,刘红露,李玉锋.应用基因芯片技术检测食品中金黄色葡萄球菌[J].食品与发酵工业,2007,33(6):108-110. |
| 作者姓名： | 何洋 周黎黎 刘红露 李玉锋 |
| 作者单位： | 1. 四川大学生命科学院,四川成都,610064;西华大学生物工程学院,四川成都,610039 2. 西华大学生物工程学院,四川成都,610039;中国测试技术研究院,四川成都,610021 3. 四川省疾病控制预防中心,四川成都,610031 4. 西华大学生物工程学院,四川成都,610039 |
| 基金项目： | 四川省应用基础研究计划;西华大学校科研和教改项目 |
| 摘 要： | 采用基因芯片技术对食品中金黄色葡萄球菌进行检测,在检测靶基因扩增后用制备的基因芯片进行杂交反应。应用基因芯片对从食品中分离的金黄色葡萄球菌进行检测,得到金黄色葡萄球菌特异性的杂交图谱,从而达到对食品中金黄色葡萄球菌进行检测的目的。实验证明,制备的基因芯片能够准确、快速检测食品中金黄色葡萄球菌。 |
| 关 键 词： | 金黄色葡萄球菌 基因芯片 16SrRNA基因 |
| 修稿时间： | 2006-12-272007-03-22 |
Applying Technology of Gene Chip to Detect Staphylococcus aureus from Foods |
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| He Yang,Zhou Lili,Liu Honglu,Li Yufeng.Applying Technology of Gene Chip to Detect Staphylococcus aureus from Foods[J].Food and Fermentation Industries,2007,33(6):108-110. | |
| Authors: | He Yang Zhou Lili Liu Honglu Li Yufeng |
| Affiliation: | 1. School of Life Science,Sichuan University, Chengdu 610064, China;2. School of Bioengineering, Xihua University, Chengdu 610039, China;3.National Institute of Measurement and Testing Technology, Chengdu 610021, China;4.Sichuan Province Centres for Diseases Prevention and Controle,Chengdu 610031 ,China |
| Abstract: | An experimental procedure is set up using the gene chip technology to detect Staphylococcus aureus from foods. Target gene was amplified and hybridized with prepared gene chip. Staphylococcus aureus were successfully discriminated from foods applying the technology of gene chip and specific hybridization maps were obtained. Staphylococcus aureus from foods were detected by technology of gene chip quickly and accurately. |
| Keywords: | Staphylococcus aureus gene ohio 16SrRNA gene |
| 本文献已被 维普 万方数据 等数据库收录！ | |