NMR microscopy of single neurons at 500 MHZ |
| |
| Authors: | Stephen J Blackband Richard W Bowtell Andrew Peters Jonathan C Sharp Peter Mansfield Edward W Hsu Nanci Aiken Anthony Horsman |
| |
| Affiliation: | (1) MR Centre, Nottingham University, NG7 2RD, Nottingham, UK;(2) Department of Biomedical Engineering, Johns Hopkins University Hospital, Baltimore, USA;(3) Department of Radiology, Johns Hopkins University Hospital, Baltimore, USA;(4) Institute of Biodiagnostics, Winnipeg, Canada;(5) NMR Department, Fox Chase Cancer Centre, Philadelphia, USA;(6) MR Centre, Hull Royal Infirmary, Anlaby Road, HU3 2JZ Hull, UK |
| |
| Abstract: | Previous NMR microimaging studies at 360 MHz have demonstrated a clear differentiation between the nucleus and cytoplasm in isolated single neurons. In particular, theT
2 of the cell nucleus is  2.5 times larger than that of the cytoplasm. In order to determine the magnitude of possibleT
2
*
influences on these observations, images of single cells have been obtained at 500 MHz using spin-echo and line-narrowing sequences. Comparison of the images acquired by the two sequences, and of the spin-echo images at 360 and 500 MHz, imply that anyT
2
*
contributions are relatively small. Consequently, the measuredT
2 differences in spin-echo imaging represent a true difference in theT
2 relaxation in the two cellular compartments. |
| |
| Keywords: | NMR microscopy single neurons T
2 measurements T 2
* measurements |
| 本文献已被 SpringerLink 等数据库收录！ |
|
