高效液相色谱法测定玉米中生育酚异构体

建立了一种玉米中生育酚异构体的测定方法。试验采用Sunfire C18反相柱（5μm4,.6×150 mm）,柱温25℃,甲醇＋水（98＋2）作为流动相,流速1.0 mL/min,进样量20μL,通过荧光检测器（激发波长为295 nm,发射波长为330 nm）测定生育酚异构体。方法线形范围为1～100μg/mL,α-生育酚（、β＋γ）-生育酚、δ-生育酚在此范围内线形良好,相关系数为0.99976～0.99983。在5～50 mg/kg浓度范围内,加标回收率为86.5%～97.4%,相对标准偏差为0.4%～3.5%。α-生育酚（、β＋γ）-生育酚、δ-生育酚的检出限分别为0.03、0.02、0.02 mg/kg。方法快速、灵敏、准确、简便易行。

Analysis of tocopherol isomers in corn by high performance liquid chromatography

A method to detecte the tocopherol isomers in corn was established.The analysis was carried out with a Sunfire C18 column(150 mm×4.6 mm I.D.,5 μm particle size) at 25 ℃.The mobile phase was methanol-water(98∶ 2,V/V).The injection volume was 20 μL,and the flow rate was 1.0 mL/min.Tocopherols were monitored at Em 295nm/Ex 330nm.The linear ranges of α-tocopherol,(β+γ)-tocopherol,and δ-tocopherol were 1～100 μg/mL(relative coefficients were 0.99976-0.99983).In the ranges of 5～50 mg/kg,the recovery rates were 86.5%～97.4%,RSDs were 0.4%～3.5%.The detection limits of α-tocopherol,(β+γ)-tocopherol,and δ-tocopherol were 0.03,0.02,and 0.02 mg/kg.In conclusion,the method is rapid,sensitive,accurate and simple.