酶联免疫吸附法测定小麦中脱氧雪腐镰刀菌烯醇

目的 建立小麦中脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)含量测定的酶联免疫吸附(ELISA)分析方法。方法 选取山东省4地区小麦样品80份, 用不同溶剂对小麦中的DON进行提取后,构建小麦中DON的ELISA检测方法, 根据国家标准对检测结果进行评价。结果 选用84%的乙腈水溶液作为提取溶剂, 其平均回收率可达100.2%, 较为理想。该ELISA方法检测小麦中DON, 线性范围是5~5000 ng/g, 回归方程Y= 0.0419X 0.0222, 相关系数r=0.9868, 样品中DON含量为5.27~3639.38 ng/g , 中位数为79.25 ng/g, 在所检测的80份样品中, 有4份超出了国家规定的上限标准(1000 ng/g), 76份符合国家规定, 合格率为95％。结论 该方法灵敏、快捷, 适用于小麦中脱氧雪腐镰刀菌烯醇的检测。

Determination of deoxynivalenol in wheat by enzyme-linked immunosorbent assay

Objective To explore a method of enzyme-linked immunosorbent assay (ELISA) for determination of deoxynivalenol(DON)in wheat. Methods A total of 80 wheat samples collected from 4 regions of Shandong province were extracted by different solvents, detected by ELISA and evaluated according to national standard. Results Optimal DON extraction was obtained when 84% acetonitrile was used in the extraction agent which could guarantee the mean extraction rate of 100.2%. The method showed a good linearity over the range of 5~5000 ng/g for deoxynivalenol and the regression equation was Y= 0.0419X 0.0222 with r=0.9868. The cotent of deoxynivalenol in wheat was 5.27~3639.38 ng/g with median of 79.25 ng/g. The concentration of 4 samples among the totals was higher than the DON tolerance limitation of 1000 ng/g, and the qualified rate was 95%. Conclusion The method is accurate and efficient and it is suitable for the determination of DON in wheat.