高通量筛选葡萄糖酸钙高产菌株

以黑曲霉为研究对象,建立了高通量筛选葡萄糖酸钙高产菌株流程。结果表明:紫外线-LiCl复合诱变处理的黑曲霉孢子悬浮液,高通量筛选得到4株高产菌株,传代后发酵性能稳定。菌株P-9,其发酵液葡萄糖酸钙含量达到23.04 g/100 mL,比原始菌种高5.86 g/100 mL。相较于传统摇瓶发酵筛选工作量大,周期长,该方法显著提高了筛选效率。

Studies on High Throughput Screening for Calcium Gluconate High Producing Strains

Using Aspergillus niger as the original strain,a high throughput method was established to screen calcium gluconate high producing strains with 48-well plates.From the spores dealed with UV-LiCl complex mutation,4 high-product strains was obtained by high throughput screening,whose fermentation efficiency was excellent after the passages of strains.According to high product of 23.04 g/100 mL for the strain of P-9,the yield was increased by 5.86 g/mL.Comparing with the traditional shake flask screening platform,this method could significantly improve the screening efficiency of calcium gluconate high producing strains.