| 三氯甲烷和乙醇对转基因成分检测的抑制 |
| |
| 引用本文： | 王东,宋君,尹全,陶李,雷绍荣,刘勇. 三氯甲烷和乙醇对转基因成分检测的抑制[J]. 中国测试技术, 2011, 0(5) |
| |
| 作者姓名： | 王东 宋君 尹全 陶李 雷绍荣 刘勇 |
| |
| 作者单位： | 四川省农业科学院分析测试中心;农业部转基因植物环境安全监督检验测试中心(成都); |
| |
| 基金项目： | 国家转基因重大课题(2008ZX08012-001); 四川省农业科学院青年基金(2009QNJJ-037) |
| |
| 摘 要： | 该文以抗除草剂(CP4-EPSPS)转基因大豆GTS-40-3-2为材料,在Lectin基因的扩增体系中设置不同浓度梯度的三氯甲烷、乙醇,模拟这2种有机试剂残留,探讨三氯甲烷、乙醇在转基因成分检测中的抑制作用。结果表明:在PCR反应体系中三氯甲烷的摩尔浓度≥3.77mol/L时,能完全抑制PCR反应;乙醇的摩尔浓度>1.39 mol/L时,PCR反应彻底被抑制。
|
| 关 键 词： | 转基因食品安全 三氯甲烷 乙醇 抑制作用 |
Inhibiting effects of chloroform and ethanol on GMO detection |
| |
| WANG Dong,,SONG Jun,YIN Quan,TAO Li,LEI Shao-rong,LIU Yong ,China). Inhibiting effects of chloroform and ethanol on GMO detection[J]. China Measurement Technology, 2011, 0(5) |
| |
| Authors: | WANG Dong SONG Jun YIN Quan TAO Li LEI Shao-rong LIU Yong China) |
| |
| Affiliation: | WANG Dong1,2,SONG Jun1,YIN Quan1,TAO Li1,LEI Shao-rong1,LIU Yong2 (1.Analysis and Test Center,Sichuan Academy of Agriculture Science,Chengdu 610066,China,2.National Center for Environmental Safety Inspection of Transgenic Plants,MOA(Chengdu),China) |
| |
| Abstract: | The different concentration of chloroform and ethanol were designed in the PCR of lectin gene of genetically modified soybean(GTS-40-3-2) in order to investigate the inhibitory effects of chloroform and ethanol on the quality PCR of genetically modified organism detection.The results showed PCR was inhibited when the concentration of chloroform was greater than or equal to 3.77 mol/L and when that of ethanol was greater than 1.39 mol/L in the reaction system. |
| |
| Keywords: | safety of GM food chloroform ethanol inhibitory effect |
| 本文献已被 CNKI 等数据库收录！ |
|
