Distribution of polyunsaturated fatty acids including conjugated linoleic acids in total and subcellular fractions from healthy and cancerous parts of human kidneys |
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Authors: | Kristina?Hoffmann J?rg?Blaudszun Claus?Brunken Wilhelm-Wolfgang?H?pker Roland?Tauber Email author" target="_blank">Hans?SteinhartEmail author |
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Affiliation: | (1) Department of Pathology, General Hospital Barmbek, 22291 Hamburg, Germany;(2) Department of Urology, General Hospital Barmbek, 22291 Hamburg, Germany;(3) Institute of Biochemistry and Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany |
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Abstract: | Differences in the FA composition of subcellular fractions from healthy and cancerous kidney tissues from the same patients
were examined. Only minor differences in CLA content were found between the healthy and the cancerous tissue portions. Regarding
the distribution pattern, CLA incorporation into nuclei and cytosol was significantly higher than incorporation into plasma
membranes and mitochondria, which could be correlated to the neutral lipid content of these fractions. The subcellular distribution
pattern of CLA was similar to that observed with monounsaturated FA but unlike that found with 18∶2n−6, which underlines the
different physiological properties of CLA and 18∶2n−6. Because PUFA have been suggested to have an effect on cancer risk,
the contents of n−3 and n−6 PUFA were determined in kidney and renal cell carcinoma (RCC). The 18∶2n−6 content and Δ5 desaturase activity were significantly lower, and the 18∶3n−6, 20∶3n−6, and 20∶5n−3 contents and Δ6 desaturase activity were significantly higher in RCC than in healthy renal tissue, indicating a changed PUFA metabolism in
RCC. Previous research has suggested that CLA inhibits the elongation and desaturation of 18∶2n−6 into 20∶4n−6. In that case,
one might speculate that a diet enriched in CLA would be a useful tool in preventing RCC. However, the involvement of CLA
in preventing renal cancer could not be demonstrated definitively from the design of this experiment. Further understanding
of the cause and/or consequence of the difference in FA metabolism may lead to a better understanding of RCC. |
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