高效液相色谱法测定大鼠全血中的灵芝酸单体Me

建立大鼠全血中灵芝酸单体Me的高效液相色谱测定方法。采用色谱柱Diamonsil~(?)C_(18)柱(250mm×4.6μm,5μm),流动相为甲醇-水(体积比为94:6,用醋酸调pH至3.6);流速为1mL/min;检测波长为245nm;内标为苯丙酸诺龙。灵芝酸单体Me血中浓度在0.05～40μg/mL范围内线性良好(r>0.99),定量限为50ng/mL,日内和日间测定的精密度(以相对标准偏差表示)均低于8.6%。该方法简便、灵敏、准确,适用于大鼠体内灵芝酸的药物动力学研究。

Determination of ganoderic acids Me in rat whole blood by high performance liquid chromatography method

An high performance liquid chromatography (HPLC) method was established for the determination of ganoderic acid Me (GA-Me) in rat whole blood. The concentration of GA-Me in blood was determined by HPLC with a Diamonsil~(?) C_(18) (250 mm×4.6 μm, 5 μm) column and a mobile phase of methanol-water (adjusted to pH 3.6 with acetic acid, 94 : 6, V/V) at a flow rate of 1mL/min. UV detector was set at 245nm. Nandrolone phenylpropionate was used as the internal standard. The calibration curve of GA-Me in blood was linear over the range of 0.05 - 40 μg/mL with a correlation coefficient of 0.9978. The lower limit of quantification was 50 ng/mL. The relative standard deviations of intra-day and inter-day determination were both less than 8.6%. This assay method is simple, precise and sensitive enough for the determination of GA-Me in blood, it can be applied to a pharmacokinetic study of GA-Me in rats.