Structural and functional characterisation of two proteolytic fragments of the bacterial protein toxin, pneumolysin |
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Authors: | PJ Morgan G Harrison PP Freestone D Crane AJ Rowe TJ Mitchell PW Andrew RJ Gilbert |
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Affiliation: | Department of Microbiology and Immunology, University of Leicester, UK. |
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Abstract: | Proteolytic cleavage of the bacterial protein toxin pneumolysin with protease K creates two fragments of 37 and 15 kDa. This paper describes the purification of these two fragments and their subsequent physical and biological characterisation. The larger fragment is directly involved in the cytolytic mechanism of this pore-forming protein, via membrane binding and self-association. The smaller fragment lacks ordered structure or discernible activity. |
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