| 农药残留生物传感器薄层电极制作工艺研究 |
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| 引用本文： | 项方献,郭康权,候俊才,伍宁丰.农药残留生物传感器薄层电极制作工艺研究[J].传感器与微系统,2009,28(5):40-42. |
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| 作者姓名： | 项方献 郭康权 候俊才 伍宁丰 |
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| 作者单位： | 1. 西北农林科技大学,机械与电子工程学院,陕西,杨陵,712100
2. 中国农业科学院,生物技术研究所,北京,100081 |
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| 摘 要： | 为了开发农药残留生物传感器的薄层电极，研究尝试采用重复银镜反应和阳极氧化法制作参比电极；以1×10^-4mol／L对硝基苯酚为测定液，采用时间-电势法对制作参比电极的工艺参数进行优化，确定Ag电极制作工艺是银镜反应次数9次、面积98mm^2，Ag/AgCl电极制作工艺是阳极氧化3min、面积70mm^2。采用循环伏安法对电极所做的检测结果表明：电极间差异小于5％，具有很好的一致性；Ag电极适用电压为-1．35—1．15V，Ag／AgCl电极适用电压为-1．45～1．0V。
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| 关 键 词： | 生物传感器 薄层电极 银镜反应 阳极氧化 |
Study on thin-layer electrode fabrication technology for pesticide residue biosensors |
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| XIANG Fang-xian,GUO Kang-quan,HOU Jun-cai,WU Ning-feng.Study on thin-layer electrode fabrication technology for pesticide residue biosensors[J].Transducer and Microsystem Technology,2009,28(5):40-42. |
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| Authors: | XIANG Fang-xian GUO Kang-quan HOU Jun-cai WU Ning-feng |
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| Affiliation: | XIANG Fang-xian1,GUO Kang-quan1,HOU Jun-cai1,WU Ning-feng2(1.College of Mechanical , Electronic Engineering,Northwest A&F University,Yangling 712100,China,2 Biotechnology Research Institute,Chinese Academy of Agriculture Science,Beijing 100081,China) |
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| Abstract: | Based on silver mirror reaction and anodic oxidation thin-layer electrode is prepared for pesticide residue biosensors. The thin-layer electrode fabrication technology parameter is optimized by time-potential determination in 1×10-4 mol/L p-nitrophenol solution. The best way to made Ag reference electrode is: silver mirror reaction 9 times, surface area of 98 mm2; Ag/AgCl electrode is fabricated by hydrochloric acid concentration anodic oxide 3 min and with the surface area of 70 mm2. Cyclic voltammetry is applied to assess the coherence of prepared thin-layer electrode. The results show that the variation is less than 5 %. The suitable voltage range is: Ag electrode of-1.35～1.15 V; Ag/AgCl electrode of-1.45～1.0 V. |
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| Keywords: | biosensor thin-layer electrode silver mirror reaction anodic oxide |
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