Evaluation of counterions for electrospray ionization mass spectral analysis of a highly sulfated carbohydrate,sucrose octasulfate |
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Authors: | Gunay Nur Sibel Tadano-Aritomi Keiko Toida Toshihiko Ishizuka Ineo Linhardt Robert J |
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Affiliation: | Division of Medicinal and Natural Products Chemistry, Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, USA. |
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Abstract: | A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other suffated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1 degree, 2 degrees, and 3 degrees ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode. |
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