Elimination of the effects of stray light in measurements by total internal reflection aqueous fluorescence (TIRAF) |
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| Authors: | D. GINGELL,& O. HEAVENS |
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| Affiliation: | Department of Anatomy &Developmental Biology, University College London, Gower Street, London WC1 6BT, U.K.;, Department of Physics, University of York, Heslington, York YO1 5DD, U.K. |
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| Abstract: | Total internal reflection aqueous fluorescence has been shown to be capable of achieving spatial resolution in surface contours of about 1 nm. When used with highly structured objects, errors in measurements can arise from light scattered either by the object or within the body of the microscope. We describe how these errors can be eliminated when studying surface contours of human platelets. |
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| Keywords: | Scatter glare fluorescence cell contact platelet total internal reflection |
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