Trifluoperazine increases fatty acid turnover in phospholipids in cultured human fibroblasts |
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Authors: | Cecile Mazière Jean-Claude Mazière Liliana Mora Martine Auclair Jacques Polonovski |
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Affiliation: | (1) Laboratoire de Chimie Biologique and U.A. 524, Faculté de Medecine Saint Antoine, 27 rue Chaligny, 75012 Paris, France |
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Abstract: | A 24-hr pretreatment of cultured human fibroblasts with trifluoperazine induced a marked increase in incorporation of saturated (stearic, palmitic) and unsaturated (oleic, arachidonic) fatty acids into phospholipids (1.5- to 2-fold for 5.10−5 M trifluoperazine). Concomitantly, incorporation into cholesteryl esters was strongly inhibited (20% of control for 5.10−5 M trifluoperazine). The drug did not change the phospholipid composition of treated cells. The effect of trifluoperazine on oleic acid incorporation into phospholipids was time-dependent and reached a maximum after a six-hr preincubation with the drug. Trifluoperazine also induced an increase in the rate of chase of oleic acid from the different phospholipid classes. In vitro preincubation of cell-free extracts with trifluoperazine resulted in activation of phospholipid acyltransferases, whereas cholesterol acyltransferase activity was decreased. The rapid effect of trifluoperazine together with its effect on a cell-free system suggests a direct action of this amphiphilic drug on the acyltransferase activities, probably by modification of the structural organization of cellular membranes. |
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