Detection of genetically modified rice: collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice |
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Authors: | Lutz Grohmann and Dietrich M?de |
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Affiliation: | (1) Bundesamt für Verbraucherschutz und Lebensmittelsicherheit, Abteilung Gentechnik, Mauerstr. 39-42, 10117 Berlin, Germany;(2) Landesamt für Verbraucherschutz Sachsen-Anhalt, Fachbereich Lebensmittelsicherheit, Freiimfelder Str. 66/68, 06112 Halle, Germany |
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Abstract: | A collaborative trial study has been conducted for validation of an extraction method and a subsequent real-time PCR for detection
of a transgenic Bt rice line (‘Bt63’) in rice products originating from China. A total of 17 laboratories participated in
the study and each laboratory received 16 coded samples comprising of rice grain flours, rice noodle flours and plasmid DNAs.
Of the accepted results all Bt63-positive rice grain samples (0.1 or 0.05% w/w) and all rice noodle samples prepared from
marketed rice products were detected correctly. The result demonstrates that ‘Bt63’ rice is detectable even at low relative
mass concentrations of 0.05%. The absolute LOD determined with plasmid DNA samples showed to be at least five copies of the
‘Bt63’ target sequence. The data provided in this study show that the method is fit-for-purpose to inspect Chinese rice products
for the presence of EU-unauthorised rice lines carrying the ‘Bt63’ construct. |
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Keywords: | Genetically modified rice Detection method Real-time PCR Collaborative trial Bt63 |
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