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宏基因组学在普洱茶微生物研究中的应用
引用本文:王 辉,李亚莉,苏丹,肖炜,周红杰*. 宏基因组学在普洱茶微生物研究中的应用[J]. 食品安全质量检测学报, 2015, 6(6): 2195-2200
作者姓名:王 辉  李亚莉  苏丹  肖炜  周红杰*
作者单位:云南农业大学龙润普洱茶学院,云南农业大学龙润普洱茶学院,云南农业大学龙润普洱茶学院,云南大学微生物研究所,云南农业大学龙润普洱茶学院
基金项目:国家自然科学基金项目(31260197, 31480215)
摘    要:宏基因组学(metagenomics)又叫微生物环境基因组学。通过从环境样品中提取全部微生物的DNA从而构建宏基因组文库,利用基因组学研究环境样品中所包含的全部微生物的遗传组成及其群落功能。它是在微生物基因组学的基础上发展起来的一种研究微生物多样性、开发新的生理活性物质(或获得新基因)的新理念和新方法。近几年宏基因技术广泛应用于微生物研究。本研究就宏基因组学应用于普洱茶微生物群落结构及其动态变化等方面进行总结,展望宏基因组学应用于普洱茶微生物研究的发展前景。

关 键 词:宏基因组学   普洱茶   微生物
收稿时间:2015-03-06
修稿时间:2015-06-05

Application of Metagenomics in Microbial Research of Pu-erh Tea
WANG Hui,LI Ya-li,SU Dan,XIAO Wei and ZHOU Hong-jie. Application of Metagenomics in Microbial Research of Pu-erh Tea[J]. Journal of Food Safety & Quality, 2015, 6(6): 2195-2200
Authors:WANG Hui  LI Ya-li  SU Dan  XIAO Wei  ZHOU Hong-jie
Affiliation:Longrun Pu-erh Tea Academy, Yunnan Agricultural University,Longrun Pu-erh Tea Academy, Yunnan Agricultural University,Longrun Pu-erh Tea Academy, Yunnan Agricultural University,Institute of Microbiology,Yunnan University and Longrun Pu-erh Tea Academy, Yunnan Agricultural University
Abstract:Metagenomics is also called microbial metagenomics. DNA of all microorganisms was extracted from environmental samples so as to establish the metagenomic library, the genetic composition and community function were studied by genomic research strategies. On the basis of the microbial genomics study, meta-genomics was developed, which was a new idea and new method to research the microbial diversity and the development of new bioactive substances (acquire new gene). In recent years, metagenomic technology is widely used in microbiological research. In this paper, the current status of research of metagenomics applied in Pu'er tea microbial community structure and its dynamic change have been summarized. And the future prospects of metagenomics that have been applied to the development of microbiological research in Pu'er tea have also been stated.
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