Enhancement of low density lipoprotein binding to both low density lipoprotein receptor-positive and- negative cells by tetracycline antibiotics |
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Authors: | Shinji Miura Keiji Hasumi Ritsuko Takayasu Maki Sugimoto Akira Endo |
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Affiliation: | (1) Department of Applied Biological Science, Tokyo Noko University, 3-5-8 Saiwaicho, 183 Fucho, Tokyo, Japan |
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Abstract: | Some tetracycline (TC) antibiotics, including TC and anhydrotetracycline, have been found to enhance specific binding of low
density lipoprotein (LDL) to both LDL receptor-positive and-negative cells at relatively higher concentrations. When incubated
at 37°C, the ability of LDL receptor-negative human fibroblasts to bind 125I-LDL was increased from<2 to 45 ng/mg by 170 μM TC. In normal human fibroblasts and Hep G2 cells, 125I-LDL binding was elevated 1.4- to 2-fold by 113 μM TC. The 125I-LDL binding in the presence of TC was diminished by both heparin and EDTA. The enhancement by TC was not observed when 125I-LDL binding was assayed at 4°C. TC enhanced LDL binding to paraformaldehyde-fixed Hep G2 cells, excluding LDL receptor induction
in the mechanism. These results demonstrated that TC enhanced cellular LDL binding through a process not involving functional
LDL receptors. |
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