| 长双歧杆菌BIOMA 5920的冻干保护剂配方的优化(英文) |
| |
| 引用本文： | 杨婵媛,朱晓丽,范代娣,米钰,骆艳娥,惠俊峰,苏然.长双歧杆菌BIOMA 5920的冻干保护剂配方的优化(英文)[J].中国化学工程学报,2012,20(5):930-936. |
| |
| 作者姓名： | 杨婵媛 朱晓丽 范代娣 米钰 骆艳娥 惠俊峰 苏然 |
| |
| 作者单位： | 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China |
| |
| 基金项目： | Supported by the National High Technology Research and Development Program of China (2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169 and 31000019);the Xi’an Research and Development Program(NC08005, YF07078);the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109);Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014);NWU Graduate Innovation and Creativity Funds (08YSY17);Shaanxi Key Subject Program, China |
| |
| 摘 要： | Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the compara- tive analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10 9 cfu·g-1 , greatly exceeding the minimum viable count requirement (10 6 cfu·g-1 ).
|
| 关 键 词： | Bifidobacterium freeze-drying viability protective agent response surface methodology |
| 收稿时间： | 2011-03-06 |
Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920 |
| |
| YANG Chanyuan,ZHU Xiaoli,FAN Daidi,MI Yu,LUO Yan’e,HUI Junfeng,and SU Ran.Optimizing the Chemical Compositions of Protective Agents for Freeze-drying Bifidobacterium longum BIOMA 5920[J].Chinese Journal of Chemical Engineering,2012,20(5):930-936. |
| |
| Authors: | YANG Chanyuan ZHU Xiaoli FAN Daidi MI Yu LUO Yan’e HUI Junfeng and SU Ran |
| |
| Affiliation: | 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;2. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;3. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Urban and Environmental Science, Northwest University, Xi'an 710069, China |
| |
| Abstract: | Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the comparative analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×109 cfu·g-1, greatly exceeding the minimum viable count requirement (106cfu·g-1). |
| |
| Keywords: | |
| 本文献已被 CNKI ScienceDirect 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载全文 |
|
