一例肝衰竭婴儿外周血红细胞超微结构改变的电镜观察

目的：观察肝衰竭婴儿红细胞形态的超微结构变化。方法：采集一例肝衰竭婴儿的静脉血,制作扫描电镜和透射电镜样品观察红细胞的超微结构。结果：在扫描电镜下,该婴儿正常双凹圆盘形红细胞数百分比仅为2.5%,而泪滴形、靶形、口形、网织红细胞、刺形、棘形和椭圆形等多种异常形态的红细胞却高达红细胞总数的97.5%。在透射电镜下,网织红细胞和少数成熟红细胞切面具有棒状突起,突起分枝或不分枝,甚至形成了高度曲折凹陷的迷宫图形。结论：该例肝衰竭婴儿红细胞形态从外形到内部超微结构均有显著改变,提示红细胞形态多样性可能与病情复杂和严重程度有关。

Electron microscopic observation of the ultrastructural changes of erythrocyte in peripheral blood of an infant with liver failure

Objective:To study the ultrastructural changes of erythrocytes in an infant with liver-failure.Methods: Venous blood sample was collected from an infant with liver failure.Erythrocytes were observed under scanning electron microscope(SEM) and transmission electron microscope(TEM).Results: Under SEM,the percentage of normal biconcave erythrocytes was only 1.5%,while various abnormal erythrocytes(include elongated erythrocytes,target cells,stomatocytes,reticulocytes,acanthocytes,echinocytes and other abnormal erythrocytes) took account for a proportion of 97.5%.Under TEM,reticulocytes and few mature erythrocytes show ropalocytosis.The club-shaped cells had branched or unbranched processes,which resulted in a maze with complex folds or deep invaginations of the cell membrane surface.Conclusions: The ultrastructure of erythrocytes was significantly altered in the infant with liver failure,suggesting that the morphosis of erythrocyte might be associated with the complexity and severity of the disease.