基于液相色谱-质谱的代谢组学方法研究卷柏治疗高尿酸血症大鼠的作用机制

采用基于超高效液相色谱-电喷雾-四极杆飞行时间质谱（UPLC-ESI-QTOF/MS）的代谢组学方法研究卷柏治疗高尿酸血症大鼠的作用机制，运用主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA）方法对健康对照组、高尿酸血症模型组和卷柏组的大鼠尿液中内源性代谢物进行分析，寻找卷柏治疗高尿酸血症大鼠的潜在生物标记物。结果表明，经PCA和OPLS-DA分析后，健康对照组、高尿酸血症模型组和卷柏组的大鼠尿液代谢物谱能得到有效地区分；发现并鉴定了9个潜在生物标记物，分别为尿酸、尿囊素、黄尿酸、犬尿酸、硫酸吲哚酚、马尿酸、肌酐、富马酸和柠檬酸。卷柏对高尿酸血症大鼠的治疗主要体现为对嘌呤代谢、色氨酸代谢、三羧酸循环、精氨酸和脯氨酸代谢以及苯丙氨酸代谢的调节作用。同时，通过检测血清生化指标，表明卷柏能降低高尿酸血症大鼠的血尿酸水平，并起到保护肾脏的作用。

Metabonomics Study ofSelaginella tamariscina for Hyperuricemia in Rats Using UPLC-ESI-QTOF/MS

A urinary metabonomics method based on ultra-performance liquid chromatography-electrospray ionization quadruple time-of-flight/mass spectrometry (UPLC-ESI-QTOF/MS) was developed to study the effects of Selaginella tamariscina on hyperuricemic rats. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were applied to analyze the metabolites in healthy control group (HCG), model group (MG) and Selaginella tamariscina treated group (STG). The results show that significant differences in urinary metabolic profiles are observed from HCG, MG and STG by using PCA and OPLS-DA. And nine potential biomarkers including uric acid, allantoin, xanthurenic acid, kynurenic acid, indoxyl sulfate, hippuric acid, creatinine, fumaric acid and citric acid are found. Pathways of purine metabolism, tryptophan metabolism, tricarboxylic acid cycle, arginine and proline metabolism and phenylalanine metabolism are in response to the therapeutic effects of Selaginella tamariscina. Besides, the serum biochemical analysis demonstrated that Selaginella tamariscina can not only reduce the amount of uric acid but also protect the kidney of hyperuricemic rat.