Characterization of dose delivery in a hard X-ray irradiation facility

The Radiation Bioengineering Laboratory at Seoul National University (SNU) operates a user-constructed hard X-ray irradiation facility for radiation biology and radiation therapy physics studies. The system package of YXLON model 450-D08 operating at the anode voltage of up to 450 kV is a key part of the facility, which enables in vitro cell irradiation and animal irradiation for in vivo studies. In this article, dose delivery in the hard X-ray irradiation facility was characterized in terms of the dose vs. operational parametric combination of the facility. The operational parameters included beam tube anode voltage, beam tube current, irradiation time, and beam exit-to-sample distance. Bremsstrahlung X-rays at energy below approximately 20 keV were filtered out by a 3 mm-thick aluminum plate fitted over the 5 mm-thick beryllium window. Gafchromic EBT films were used as radiation sensor materials in dose measurement. The characterization was validated via experimental observation of the in vitro biological responses of cells to radiation exposure. The biological responses obtained using the new hard X-ray irradiator were highly comparable with those obtained using a commercial gamma-ray irradiator.