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Sodium Dodecylsulphate Electrophoretic Demonstration of Selective Adsorption of Gelatin Polypeptides on Silver Halide Particles-A Qualitative Examination
Authors:Shohei Aoyagi  Yoshiya Shima  Hirotaka Shimanouchi
Affiliation:1. Faculty of Engineering, Chiba University, 1-33 Yayoi-cho, Chiba, 260, Japan;2. Department of Life Science, Faculty of Science, Toko Tnstitute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 227, Japan
Abstract:The gelatin fraction adsorbed on silver iodobromide particles was recovered by dissolution of the particles with sodium thiosulphate. The sodium dodecylsulphate, SDS, electrophoretic pattern of the recovered fraction was clearly different from that of the original gelatin. This is a direct indication of selective adsorption. Extraction of the adsorbed gelatin from the particles was examined with several reagents. Among them, the SDS extract gave a similar electrophoretic pattern to that of the fixation-recovered fraction. Electrophoretic patterns of SDS-extracted fractions from AgCl, AgBr and Agl particles indicate that the absorptions by these silver halides are selective and selectivity by each silver halide is different.
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