Depigmenting effect of <Emphasis Type="Italic">Sterculia lynchnophera</Emphasis> on B16F10 melanoma and C57BL/6 melan-a cells |
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Authors: | Uyen Do Phuong Lam Dung Nguyen Hoang Hyang-Bok Lee Bora Kim Joo-Dong Lee Jeong-Hyun Shin Eun-Ki Kim |
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Affiliation: | (1) R & D Center, AmorePacific Corporation, 314-1 Bora-Dong, Giheung-gu, Yongin, 446-729, Korea;(2) Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 446-701, Korea;(3) Skin Biotechnology Center, Kyung Hee University, #1, Seocheon-dong, Giheung-gu, Yongin-si, 446-701, Korea; |
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Abstract: | To develop a novel skin depigmenting agent from natural sources, the inhibition of melanogenesis by the Chinese herb, Sterculia lynchnophera (SL), was evaluated. Treatment of B16F10 melanoma cells and melan-a cells with SL exhibited a 32.9% and 68.2% inhibition
of melanin synthesis without cytotoxicity at a concentration of 200 μg/ml, respectively. This herb possessed a high free radical
scavenging activity with IC50=11.02 μM. The methanol extract of SL slightly inhibited in vitro mushroom tyrosinase activity (23.4% at a concentration of 200 μg/ml) and had a significant inhibitory effect on cellular
tyrosinase activivity (48.65% and 88.56% inhibition at the concentration 200 μg/ml in B16F10 cells and C57BL/6 melan-a cells,
respectively). From the western blotting results, SL inhibited the expression of tyrosinase and tyrosinase related protein
1 (TRP-1). Taken together, we suggest that SL may be a safe and effective depigmentation agent. |
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