野生和种植藜蒿不同部位提取物的抗氧化活性和酶抑制活性

藜蒿为江西特色食物资源,其嫩茎常被加工成美食,而叶和根被作废弃物直接丢弃。本研究比较了野生藜蒿叶、茎和根及种植藜蒿叶、茎的总酚和总黄酮含量以及提取物的抗氧化、α-葡萄糖苷酶、黄嘌呤氧化酶和乙酰胆碱脂酶活性抑制能力,旨在为藜蒿副产物的高值化利用提供参考。结果表明,野生和种植藜蒿叶分别含有最高的总酚含量(30.12 mg GAE/g DM)和总黄酮含量(0.63 mg QuE/g DM)。野生藜蒿叶提取物的DPPH·清除能力、ABTS^+·清除能力和乙酰胆碱酯酶抑制能力最强,其IC50值分别为0.37,0.39 mg DM/mL和10.06 mg DM/mL。野生藜蒿根提取物的α-葡萄糖苷酶和黄嘌呤氧化酶活性抑制能力最强,其IC50值分别为14.08 mg DM/mL和4.01 mg DM/mL,其次为野生藜蒿叶。相关性分析表明,酚类化合物是藜蒿中最主要的抗氧化活性成分。相比于种植藜蒿和野生藜蒿茎,野生藜蒿叶是天然抗氧剂、α-葡萄糖苷酶抑制剂、黄嘌呤氧化酶抑制剂和乙酰胆碱酯酶抑制剂的更优质的原料来源,具有进一步研究其抗糖尿病、高尿酸血症和老年痴呆症的价值。

Antioxidant and Enzyme Inhibitory Activities of Extracts from Various Parts of Wild and Cultivated Artemisia selengensis Turcz

Artemisia selengnesis Turcz(AST)is a special food resource of Jiangxi province,and the tender stems of it are often processed into delicious food,but their leaves and root are discarded as directly.This study compared the content of phenolics and flavonoids,antioxidant activity,acetylcholinesterase inhibition,α-glucosidase and xanthine oxidase inhibition of wild and cultivated AST leaves,stems and wild AST root,to aim to provide information for high value utilization of by-products of AST.The results showed that wild and cultivated AST leaves had the higest phenolics(30.12 mg GAE/g DM)and flavonoids(0.63 mg QuE/g DM),respectively.What’s more,wild AST leaf extract gave the strongest DPPH·scavenging ability,ABTS^+·scavenging ability and acetylcholinesterase inhibition,the IC50 value was 0.37,0.39 and 4.01 mg DM/mL,respectively.The strongestα-glucosidase and xanthine oxidase inhibition was observed in wild AST root extract with the IC50 values of 14.08 and 4.01 mg DM/mL,and the second is wild AST leaf.Correlation analysis indicated that phenolics was the major antioxidant components of AST.Thus,compered to cultivated AST and wild AST stems,wild AST leaves are more suitable as natural resourse of antioxidants,α-glucosidase,acetylcholinesterase and xanthine oxidase inhibitors,and there should have a further study on antidiabetic,anti-hyperuricemia,anti-Alzheimer’s disease effect of it.